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Visualization of the bound antibodies was carried out by adding 200 L of mixed substrate solution and incubating the slides for 510 min at room temperature

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Determination of mannose released from the cell wall is a procedure used by several authors as an indicative value of mannoprotein release during yeast autolysis ( FIGURE 6 Along incubation time, the concentration of mannose in the medium in which the yeast cells were suspended was higher in the biomass that had been previously treated by PEF