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The combined TMT-labeled peptide sample was fractionated by basic reverse phase (bRP) fractionation using an in-house packed SDB-RPS (3M) StageTip

While systemic absorption is minimal, topical delivery ensures the concentration is highest at the site of concern (pigmentation, dark spots), leading to excellent localized brightening effects

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Disadvantages involve benefit decline during off periods requiring acceptance of fluctuation, complexity planning and tracking cycles, uncertainty about optimal timing and duration for individual responses, and potential for forgetting to restart after extended breaks

doi: 10.1016/j.biortech.2022.128030 53 PereiraL.CotasJ