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After five days in expansion medium, monolayers were fully confluent and were switched to differentiation medium, consisting of the same recipe as expansion medium but omitting nicotinamide, SB202190, and Wnt surrogate

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Summary Keywords stress response, redox homeostasis, transcription, purine nucleotide, glutathione Citation Mitsuishi Y, Motohashi H and Yamamoto M (2012) The Keap1Nrf2 system in cancers: stress response and anabolic metabolism