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The composition of the internal pipette solution was: 115 mM CsMeSO 3 , 20 mM tetraethylammonium chloride, 10 mM CsCl, 10 mM HEPES, 5 mM NaCl, 0.6 mM EGTA, 4 mM Mg-ATP, 0.3 mM Na2GTP and 10 mM QX-314 (lidocaine N-ethyl bromide)

Resulting plots were generated in the R statistical computing environment using standard libraries, including ggplot2 for general plotting, factoextra for Principal Component Analysis, corrplot for correlation matrices, and pheatmap for Hierarchical Clustering

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Wait 1-2 minutes between layers