Local resolution was calculated in RELION
Haematoxylin (HT-109, Sigma Aldrich) was used for counterstaining
Disruptions to core clock genes including Per2, Clock , and NPAS2 display significant reductions in glutamate uptake, mRNA expression of Glast, an astrocyte-specific glutamate transporter, and protein expression (Sofroniew, 2015)
MSCs are typically obtained from bone marrow, adipose tissue, and more recently, umbilical cord tissue, each offering unique benefits concerning harvesting ease, differentiation capabilities, immune compatibility, and ethical implications
Instead of targeting GHRH receptors, it binds to Growth Hormone Secretagogue Receptors (GHS-R1a) , commonly known as ghrelin receptors