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It is challenging to reconcile these gene changes with our behavioral findings since they occurred in both sexes while recognition memory was only affected in alcohol-exposed males

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Repeat under similar conditions if the first result is borderline or surprising

Strategies to minimize reactions include rotating injection sites consistently to avoid repeated trauma to the same area, ensuring the solution is at room temperature before injection (cold injections can increase irritation), injecting slowly rather than quickly, and using a smaller gauge needle for less tissue trauma