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"After comparison with this new low-temperature data, it seems evident that the best fit of a single [ammonium sulfide] ice is one that has been irradiated and warmed up to higher temperatures so as to remove the [sulfur] radical," the researchers said

However, due to the brains high oxygen consumption and lipid-rich environment, it is particularly vulnerable to oxidative stress when ROS levels exceed antioxidant defenses

Clin Exp Pharmacol Physiol (2013) 40(7):42230

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At the end of 16 weeks, the animals were fasted overnight and then euthanized by exposure to isoflurane (Webster Veterinary Supply Inc., Devens, MA)