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Briefly, 2030 oocytes were lysed on ice in mammalian lysis buffer 5X (ab179835, Abcam), then deproteinized, neutralized, and diluted to concentrations suitable for standard curve fitting

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NR has emerged as a leading NAD + precursor candidate due to its high bioavailability, its safety in healthy adults [3, 31, 48] or obese patients [9], and its superiority in efficient NAD + elevation in humans as compared to other agents [48]

however, it needs building blocks for repair either from supplementation or diet

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