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After blocking with 5% FBS (for GPX4, KIM-1 and cleaved caspase-3) or 10% FBS (for 4HNE) in Tris-buffered saline, pH 7.4 containing 0.01% Tween-20 for 30 min, the sections were incubated with the primary antibodies (anti-GPX4 1:100

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Older adults : As we age, our bodies become less efficient at absorbing nutrients like B12, and deficiency is more common after age 60

There is also a lot talk around other antioxidants like vitamin C and vitamin D helping these conditions