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206 In addition, expression of the mitochondrial isoform of phosphoenolpyruvate carboxykinase, involved in gluconeogenesis from non-carbohydrate carbon substrates such as pyruvate, lactate, glycerol and glucogenic amino acids, was significantly increased, 499 indicating an elevated demand for free glucose through enhancing astrocytic glucose metabolism in GDI1 -null mice

Elevated serum B12 levels often indicate underlying conditions such as haematological disorders, liver disease, or renal impairment rather than toxicity from supplementation

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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s