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Mechanistic Rationale NNMT consumes nicotinamide and SAM to produce 1-MNA and S-adenosylhomocysteine (SAH)

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Hydrogen atoms were added using Chimera 1.14 32

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Inhibition activities were measured as described ( Inhibition of Microsomal Prostaglandin E 2 Synthase 1 (mPGES-1) in a Cell-Free Assay The enzyme activity of human recombinant microsomal prostaglandin E 2 synthase 1 (mPGES-1) was measured by assessment of PGH 2 conversion to PGE 2 ( 2 was added to start the reaction