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Moreover, it caused nuclear p53 exclusion and induced apoptosis selectively in senescent Leydig cells by disrupting the FOXO4-p53 interaction, which may be a critical mechanism underlying its therapeutic effect

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Briefly, 400 m slices were cultured on (Milipore, Billerica, Mass.) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, Calif.), according to the manufacturer's protocol, to visualize dendritic arbors