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SAM is additionally required for phosphatidylcholine synthesis, which is abundant in neuronal membranes and is crucial for synaptogenesis and neurite outgrowth ( Vitamin B12 is a cofactor for the enzyme methylmalonyl-CoA mutase, which converts methylmalonyl-CoA to succinyl-CoA, a crucial step in fatty acid metabolism (73)

The next step involves the enzyme glutathione synthetase, responsible for adding glycine to the dipeptide to produce GSH (-glutamylcysteinylglycine) and also requires coupled ATP hydrolysis (Meister, 1974)

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Studies have found that the combination of L-theanine, Magnolia officinalis , and Melissa officinalis showed neuroprotective effects against excitotoxicity in vitro and anti-anxiety and antidepressant activities in vivo [190]