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Methods Original and review articles encompassing the connections between insulin/IGF-1 and STAT3 signaling were examined from major databases including Pubmed, Scopus, and Google Scholar

J Cell Biol 172:151162 Burt CM, Beller JP, Abshir MY, Collins JS, McCartney CR, Marshall JC (2012) Neuroendocrine dysfunction in polycystic ovary syndrome

Proteins in the samples were precipitated by boiling or treating with 10 % cold perchloric acid and then centrifuging at 4 C and 12,000 rpm for 10 min

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However, the total radioactivity in (lactate + pyruvate + carbon dioxide) is ~14090 dpm in all cases - from the initial specific activity this gives a rate of glucose utilisation of 640 nmol /mL cells /hour