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and/or (iii) Diagnostic Statistical Manual diagnosis by a physician along with standardized validated questionnaires which have good correspondence to the gold-standard instruments including the Social Responsiveness Scale (SRS) [34, 35], the Social Communication Questionnaire [36,37,38] and the Autism Symptoms Questionnaire [39] and diagnosis confirmation by the Principal Investigator (first author) who specializes in the diagnosis and treatment of children with ASD

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Elevated NAD+ levels activate sirtuins, particularly SIRT1, which are NAD+-dependent deacetylases that regulate numerous metabolic processes including fat oxidation, glucose metabolism, mitochondrial biogenesis, and cellular stress responses

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