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coli strain MG1655 was used as the starting strain to implement metabolic engineering

An excess of either can reduce the absorption of the other

Lentiviral gene transduction The shRNA sequence against SRD5A1, that is CTCGAGTGCTGTTGACAGTGAGCGACCTGTACCTGTTATCAATATATAGTGAAGCCACAGATGTATATATTGATAACAGGTACAGGCTGCCTACTGCCTCGGAGAATTC, was cloned into TRIPZ vector (Thermo Fisher Scientific, USA), which provides inducible shRNA expression in the presence of doxycycline (Dox)

For intravenous vitamin B12, the usual dose is between 500 mcg and 1 mg

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