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Detection of high proliferative minimal residual disease in ALL patients with AML and MDS in complete remission with monosomy 7 by FACS/FISH

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Trono (Ecole Polytechnique Federale de Lausanne, Lausanne, Swisse)] and MISSION pLKO.1-puro-based vectors (Sigma-Aldrich, Madrid, Spain,) encoding either a non-targeting shRNA (SHC002) or GCLC -targeting shRNAs sh-62 (TRCN0000344862), sh-65 (TRCN0000333565), and sh-86 (TRCN0000048486)

To reduce the number of steps in a sample preparation procedure a simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine (TCEP) and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide (BCPB) has been applied