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However, the percentage varies with every injection

Elution was performed with 5 mM H 2 SO 4 at 60 C and a flow rate of 0.6 mL/min, using a refractive index detector for detection

Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)

This specific configuration, driven by combined hydrophilic and ionic interactions, ensures excellent retention, separation, and symmetrical peak shapes for all critical polar analytes without any prior chemical modification

Ousingsawat, J., Kongsuphol, P., Schreiber, R