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suggests that glutathione represents an electron donor in the event of vitamin C regeneration

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We also examined the role of glycosylation by introducing asparagine-to-alanine substitutions at six different potential N -glycosylation sites in surface-targeted LacAnc100, none of which appeared to notably reduce its activity (Supplementary Fig

^ Neurodegenerative disorders in humans: the role of glutathione in oxidative stress-mediated neuronal death

Then, 10 l of LacAnc100 or LaccID was added to flat-bottom 96-well plates (Greiner Bio-One, 655101) and 190 l of reaction mixtures containing PBS and different substrates were added with the help of a Multidrop system (Multidrop Combi, Thermo Scientific)