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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h

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The function of the guiding bar, as postulated in our previous report 16 , could be strengthened by the crystal structure, and disruption of the guiding bar led to an increase in Trx-reduction efficiency, which further supports its importance for regulatory processes in catalysis of TrxR1