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Ultrasonic Imaging and Measurements The ultrasound examination was performed in rats (either deeply anesthetized for immediate post-surgery assessment or deeply sedated for assessment in later periods) with a portable ultrasound device (Philips Healthcare, Lumify, The Netherlands) by measuring the distance (in mm) of the separated muscle from the anatomical origin at the iliac and femoral bones, where the samples were placed in a predefined repeatable position, in the right lateral decubitus position with the knee and hip in full extension and both joints in the 90 flexion position

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Both routes are effective, though subcutaneous injection has become more popular due to ease and comfort

The studies tested various doses including 0.25 mg, 0.5 mg, and 1 mg daily via subcutaneous administration over periods ranging from single doses to 24 weeks of continuous therapy

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