The cumulative effect is elevated oxidative stress within ovarian follicles, as evidenced by increased malondialdehyde (MDA) levels in ovarian homogenates of HFD-fed murine models ( Different studies underscored the role of adipose-derived inflammatory mediators in driving oxidative stress under HFD conditions, linking them to follicular dysfunction and a reduced ovarian reserve ( Additional studies also report that chronic inflammation impairs key intracellular signaling cascades, such as PI3K/Akt and mTOR, in granulosa cells, thereby promoting ROS accumulation and impairing follicular maturation ( Altogether, these findings indicate that HFD-induced metabolic inflammation is a central mechanism underlying ROS-mediated ovarian damage
Recommended Dilution Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
[DOI] [PMC free article] [PubMed] [Google Scholar] 152.Gong W., Guo P., Li Y., Liu L., Yan R., Liu S., Wang S., Xue F., Zhou X., Yuan Z
Studies have indicated that changes in the ratio of Firmicutes to Bacteroidetes might affect lipid metabolism and the central nervous system, potentially leading to obesity (Ley et al., 2005
PD, Parkinsons Disease
