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Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)

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2 Materials and methods 2.1 Cell lines We used MEC-1 cells from a CD5 low / CLL cell line established from a patient with CLL in transformation to B-cell prolymphocytic leukemia (generously provided by Dr

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