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Phosphorylation of MCU enhances mitochondrial Ca 2+ uptake, precipitating mitochondrial Ca 2+ overload, opening of the mitochondrial permeability transition pore (mPTP), loss of m, release of pro-apoptotic factors and progressive mitochondrial dysfunction ( 3.2 The impact of oxidative stress on the cryopreservation of ovarian tissue The cryopreservation and transplantation of ovarian tissue are not constrained by the patients age or timing