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Induction of shear stress by exposure to fluid movement decreased apoptosis, enhanced proliferation, and the mobility of endothelial cells in these models but did not alter the expression of TJ proteins or P-gp (DeStefano et al., 2018)

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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)