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A thorough pre-treatment conversation should include baseline biomarker assessment, rule-out of reversible causes of hair loss (nutritional deficiency, thyroid disease, hormonal imbalance), and realistic expectations about evidence

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The lower K D value determined from competition with His might arise due to the omitting of the formation of a putative ternary complex between HSA, Cu(II) ion and His in the binding scheme, which should result in higher affinity estimates, since the copper in the ternary complex is found in the HMW peak