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Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

However, the prospective Shanghai Osteoarthritis Cohort study showed that GLP-1RA users with KOA and diabetes exhibited a greater reduction in Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total and pain score and a lower occurrence of knee surgery compared to controls

You're saving two ATP, which is the energy source, and one NAD