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Data-dependent acquisition (DDA) scans were acquired based on a cycle time of 1.3 s, a resolution of 15.000 at m/z 200, isolation window 1.2 m/z, normalized stepped collision energies 17, 27, 37%, AGC target 100%, maximum injection time 60 ms, dynamic exclusion for 6 s after two-times fragmentation within 6 s

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