The cells treated for 1 h with 200 M of the ROS inducer TBHP were used as a positive control
Measurement of GSSG with or without GSH masking reagent Interference Reducing agents such as ascorbic acid, -mercaptoethanol, dithiothreitol (DTT) and cysteine, or thiol reactive compounds such as maleimide compounds, interfere with the glutathione assay
[DOI] [PMC free article] [PubMed] [Google Scholar] 207.Moling O., Cairon E., Rimenti G., Rizza F., Prister R., Mian P
Why Peter chose a paper on metformin, how metformin works, and why it generated excitement as a longevity-enhancing agent [9:00]
24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants