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Nat Med 11:13061313

GHK and GHK-PEG induce fibroblast proliferation Prior to assessing the effects of GHK and GHK-PEG on apoptosis in SH-SY5Y neuroblastoma cells, it was necessary to ensure that both peptides stimulated fibroblast cell proliferation as previously demonstrated by a number of studies

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Western blotting Cell or tissue samples were lysed with RIPA buffer containing 1% protease inhibitor, and the protein concentration of cell lysates was measured by BCA protein assay kit (Beyotime, China)