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The Nrf2-Antioxidant Response Element Signaling Pathway and Its Activation by Oxidative Stress

Twenty grams were blended for 2 minutes with cold chloroform-methanol mixture (2:1 v v -1 ) in proportion of 10 mL of solvent mixture per gram of tissue (except 20 ml of solvent per gram of liver tissue) in a Warring blender

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doi: 10.1042/bj2200553 Middlehurst, CR, King, GF, Beilharz, GR, Hunt, GE, Johnson, Gfs and Kuchel, PW (1984)

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