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Then Dulbeccos Modified Eagle Medium (DMEM, Thermo Fisher, MA, USA) containing 100 U/ml collagenase type (Sigma, MO, USA) and 15 mM HEPES (Shanghai Shenggong Co., Ltd., Shanghai, China) was utilized to perfuse the liver for an additional five minutes

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To confirm the specificity of this effect, shRNA targeting of GGT also decreased glutamate levels (Fig

Proline concentration was determined on the basis of a standard curve (050 g mL), and the value was calculated using the formula: mole proline/g FW = [(g proline/mLmL toluene) / 115.5 g/mole] / [(g sample) /5] Hydrogen peroxide content in soybean leaves was determined according to Velikova et al

AM404 acts on the transient receptor potential vanilloid 1 (TRPV1) and cannabinoid 1 (CB1) receptors in the midbrain and medulla (Roberts et al., 2002