(3) Add 300 L of chloroform, vortex for 30 s, ultrasonic extraction for 10 min, leave at -20C for 20 min, centrifuge for 10 min (12000 rpm)
Cellini B, Bertoldi M, Montioli R, Paiardini A, Borri Voltattorni C (2007) Human wild-type alanine:glyoxylate aminotransferase and its naturally occurring G82E variant: functional properties and physiological implications
Cui, W., Guo, M., Liu, D., Xiao, P., Yang, C., Huang, H., Liang, C., Yang, Y., Fu, X., Zhang, Y., Liu, J., Shi, S., Cong, J., Han, Z., Xu, Y., Du, L., Yin, C., Zhang, Y., Sun, J., & Chu, B
Instead, it works downstream to protect cells from the damage that often results from metabolic activity
Uspenski Iu, P., et al., [Device laboratory and postmortem parallels in alcoholic hepatitis during combined therapy using thioctic (alpha-lipoic) acid]