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Two tests dominate purity verification: HPLC (high-performance liquid chromatography) separates the components of a sample and quantifies the percentage of the total peak area attributable to the target compound

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Materials and Methods Materials Lactose monohydrate, trehalose, L-leucine, L-arginine, sodium carbonate, sodium bicarbonate, citric acid, sodium citrate, theobromine, ethanol, and acetonitrile (HPLC grade), and trifluoracetic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA)