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This storage protocol helps protect peptide stability and supports long-term integrity until use in research environments

Age-related NAD+ decline impairs this critical DNA repair pathway CD38 balance: The ectoenzyme CD38 consumes NAD+ and increases with age, creating a vicious cycle of NAD+ depletion and immune dysregulation By restoring NAD+ levels, supplementation recalibrates multiple aging pathways simultaneously: improved DNA repair, enhanced mitochondrial biogenesis, restored epigenetic regulation, activated autophagy, and improved nutrient sensing

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After the polymerase chain reaction (PCR), its products were determined by 1% agarose gel electrophoresis, the target band was cut and purified, and the recovered products were used for Sanger sequencing