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Neuropathol Appl Neurobiol

Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h

Monoamine reuptake modulation (Tesofensine): a small molecule originally studied in central-nervous-system research, of interest for its monoaminergic mechanism

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If the solution appears cloudy, contains floating specks, or shows any color change, the vial may be compromised and should not be used