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The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)
Currently, senolytics and their potential to enhance longevity and promote cellular rejuvenation remain a subject of active debate (Bussian et al., 2018
International Journal of Trichology , 9(3), 129134
Continued promotion of research in ferrology and the establishment of this emerging disciplinary framework will significantly facilitate the interdisciplinary exchange within the field of iron research, further advancing the prevention and control of diseases and the maintenance of human health, thereby providing essential scientific support
However, further research is necessary to substantiate the protective effects of E2 and to elucidate the specific molecular regulatory mechanisms involved, which could lead to the development of additional therapeutic strategies
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