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Cells were then washed twice with PBS and solvent (75 mM ammonium carbonate, pH was adjusted to 7.4 with acetic acid)

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Opportunities in somatostatin research: biological, chemical and therapeutic aspects

Duarte-Silva S, Silva-Fernandes A, Neves-Carvalho A, Soares-Cunha C, Teixeira-Castro A, Maciel P

A healthcare professional should administer your first shot so they can monitor your reaction and check for any adverse effects