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Following 3 10min washes with 1ml 0,2% TBS-Gelatin and 1 wash with regular TBS the slides were mounted using soft set mounting medium with DAPI (Vectashield) and sealed with nail polish

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This is a Fenton-adjacent reaction: the reduced copper(I) then reacts with any hydrogen peroxide present (generated enzymatically at the skin surface) to produce hydroxyl radicals, among the most damaging reactive oxygen species in biology