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Peptide Vials (CJC-1295 NO DAC + Ipamorelin, 10 mg blend each): 8 weeks 3 vials 12 weeks 4 vials 16 weeks 5 vials Insulin Syringes (U-100, 30- or 50-unit preferred for low volumes): Per week: 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes 16 weeks: 112 syringes Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution

The vital dye 2-(4-(dimethylamino)styryl)- N -ethylpyridinium iodide (DASPEI) (Life Technologies, Grand Island, NY) is a marker of mitochondrial membrane potential and preferentially stains lateral line hair cells when added to the surrounding EM (Harris et al., 2000) per fish were assessed based on fluorescent intensity (Harris et al., 2009)

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Between studies, the anatomical definition of ACC voxel was variable, as discussed in our prior work (7)