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Gut inflammation activates vagal afferents that signal distress to the brain, while healthy gut signaling promotes parasympathetic tone and emotional equilibrium

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All analytes were separated on an ACQUITYUPLC BEH Amide column (2.1 mm 100 mm, 130 , 1.7 m, Waters Corporation, Milford, Massachusetts) with an upstream Van Guard UPLC BEH Amide pre-column (2.1 mm 5 mm, 1.7 m, Waters Co., Milford, MA) using water and acetonitrile/water as mobile phases