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Western Blotting Analysis Proteins from the whole kidney lysates were separated on a 10% SDS-polyacrylamide gel, transferred onto PVDF membrane, blocked in 5% BSA for 60min, and then primary antibodies against KIM-1, IL-1, TNF-, Ferritin, FPN, xCT, and p53 at the dilution of 1:1,000, as well as 4-HNE, GPX4, and -actin at the dilution of 1:4,000 were probed at 4C overnight