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B12 is essential for the methylation reactions required to produce the lipids and proteins that constitute myelin

In these cases, sustained high nitrogen stress and resulting NO and polyamine up-regulation have mostly toxic effects, exacerbating both pathology and symptoms of the disease state (Bensemain et al., 2009

After rinsing, the sections were incubated in 3% hydrogen peroxide for 15 min and then incubated with 5% bovine serum albumin blocking solution at room temperature for 15 min

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