This is particularly relevant for vegetarians, vegans, and older adults who are at a higher risk of deficiency due to their dietary choices or absorption issues
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Because B12 is a water-soluble vitamin, the body typically uses what it needs and excretes the rest
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Another concept is to measure B12 bound to the plasma protein transcobalamin (holoTC, active B12) before and after administration of three high physiological doses of B12 for two days