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Al-Zaidy SA, Sahenk Z, Rodino-Klapac LR, Kaspar B, Mendell JR
These results suggest that bTNF- induces a metabolic change in bFLS, shifting from OXPHOS to aerobic glycolysis, with an increase in lactate levels, which could contribute in part to the inflammatory effect mediated by bTNF-

Step-by-Step Protocol: How to Perform a DARTS Assay Top Advantages of Using DARTS for Drug Target Identification DARTS vs CETSA: Choosing the Right Assay for Target Validation How to Analyze Protein-Protein Interaction: Top Lab Techniques AP-MS for Protein Interaction Mapping: From Pull-Down to Identification Proximity Labeling (BioID/TurboID) vs Conventional Pull-Down: Selecting the Right Interactomics Approach RNA Pull-Down and Identification of RNA-Binding Proteins: A Step-by-Step Methodology GST and Co-Immunoprecipitation Workflows: Optimizing Capture Strategies for Interactome Studies Surface Plasmon Resonance: Principles, Instrumentation, and Experimental Design for Molecular Interaction Analysis Difference Between Exogenous and Endogenous Protein Crosslinking Intracellular Protein Crosslinking Detection Methods Workflow and Application of Drug Affinity Responsive Target Stability (DARTS) Protein-Protein Interaction Identification MethodCo-immunoprecipitation (Co-IP) and Pull-Down Applications of Co-IP in Protein Interaction Studies Co-immunoprecipitation (Co-IP) Overview Co-IP Protocol-How To Conduct A Co-IP Difference between Immunoprecipitation and Co-immunoprecipitation Co-immunoprecipitation Mass Spectrometry: Unraveling Protein Interactions Protein Interactions: Techniques and Significance IP-MS Data Analysis IP-MS Sample Preparation Guidelines Materials Selection for IP-MS Experiments Proximity Labeling Techniques: Investigating Weak or Transient Protein Interactions BioID vs TurboID: A Decision Framework for Proximity Labeling Experimental Design Protein-Protein Interactions: Insights & Applications Revealing Protein Interactions in Cancer with BioID Technology Applications of BioID in Protein Interaction Studies Applications of TurboID Technology in Disease Research What is Label Transfer Protein Interaction
