The methodological details for HPLC elution and electrochemical detection of free unbound GSH, GSSG, 3-NT and 3-CT have been described previously
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To address the role of the activator candidate for glutaredoxin catalysis, we replaced Lys105 of ScGrx7 by site-directed mutagenesis
Moreover, when moderately induced by a 100 ng/ml doxycycline, these two mutations resulted in reduced GM130-LYSET colocalization and mislocalization of some LYSET to the plasma membrane in SKMEL30 cells (Fig
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