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(2) Add two small steel beads, pre-cool at -20C for 2 min, and add to a grinder (60 Hz, 2 min)

Conventional reversed-phase and mixed-mode columns lacked sufficient retention, while bare silica columns operated in HILIC mode provided strong retention but suffered from broad peaks under tested conditions

Hippocampal ROS levels and protein kinase C activity were assessed, as ROS are known activators of PKC [54]

Maintenance : After achieving the desired results, maintenance sessions every 1-2 months are recommended to sustain benefits