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The procedure can last from 30 minutes to two hours, depending on the area treated

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To further explore the effect of SP on the osteoclastogenesis in BMMs, the expressions of critical osteoclastogenesis-related genes like nuclear factor of activated T cells 1 ( NFATc1 ), c-Fos proto-oncogene ( c-Fos ), and cathepsin K ( CTSK ) were analyzed by RT-qPCR