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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)
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The original characterization by the Sikiri group framed the larger parent protein in human gastric juice as a protective factor for the gastrointestinal mucosa, with body protection compound (BPC) referring to that broader protective role
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