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GSH depletion under stress may result from either the neutralization of ROS or an elevated requirement for glutamate precursors (67)

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High-performance liquid chromatography quantification of GSH/GSSG, 3-NT and 3-CT To evaluate biomarkers of oxidative stress and damage by high-performance liquid chromatography (HPLC), approximately 200 mg of tissue was minced and homogenized in 500 l of ice-cold phosphate-buffered saline

6c), indicating that H 2 O 2 catalyzed by NP4 not only disrupted the redox balance within tumor cells but also diffused outside the cells, leading to more cascading reactions